The InSitu Porator™ is a powerful and novel tool for the examination of Intercellular Gap Junction and Signal Transduction studies performed on adherent cells grown in situ prior to the electroporation.
Following the pulse application the transient molecule i.e. Lucifer Yellow is introduced into the cell via transient pores, where its migration into adjacent non-electroporated cells can be monitored under a microscope using fluorescent illumination. The transfer distance can be precisely quantitated within a large number of cells without any detectable disturbance to the cellular metabolism.
Disposable chambers for zero cell disturbance
Non Traumatic conditions
Wide variety of Adherent Cells tested in situ
Easily optimised to create highest efficiencies
Multiwell format for parallel studies
Sterile environment to grow and monitor the cells
Fluorescent/Phase Contrast measurement in situ